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KMID : 0350519940470010515
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Journal of Catholic Medical College
1994 Volume.47 No. 1 p.515 ~ p.525
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Detection of Epstein-Barr Virus Genes and Gene products in Nasopharyngeal Carcinoma
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Abstract
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Epstein-Barr virus(EBV) is associated with infectious mononucleosis, Burkitt's lymphoma, Hodgkin's disease and undifferentiated nasopharyngeal carcinoma(NPC). The EBV-encoded RNAs (EBERs; EBER1 & EBER2), two latent gene products, are the most
abundant
RNAs expressed invitro, but their function and the significance of the intranuclear location are not established. Latent membrane protein-1(LMP-1) is an EBV latent gene product and the expression of LMP-1 in epithelial cells transforms them
morphologically.
Many studies on the relationship between EBV and NPC using the serology, the hybridization technique of nucleic acid or polymerase chain reaction(PCR) were performed.
The purpose of this study is to detect EBV in nasopharyngeal carcinoma and to analyze the cxpression and the localization of EBV gene products at the cellular level. The authors analyzed paraffin embedded tumor samples from 40 cases of
nasopharyngeal
carcinoma using EBERs probe set(cDNA oligonucleotide probe for EBERs) by DNA-RNA hybridization and LMP specific monoclonal IgG antibodies, CS1-4, by immunohistochemical stain.
@ES The results were as follows:
@EN 1) Mean age of patients with nasopharyngeal carcinoma was 52.3, and the sex ratio between the male and the female was 4.7:1. The lvmphocytic infiltration is more abundant in undifferentiated carcinoma than keratinizing squamous cell carcinoma
or
nonkeratinizing carcinoma (X*=16.97 P=0.026).
2) The EBERs were demonstrated in the malignant epithelial cells from 23(57.5%) of 40 patients with nasopharyngeal carcinoma. Recognition of EBERs expression was facilitated by its characteristic location in the nucleus with nucleolar sparing.
3) The EBERs detection rate in the male was significantly higher than in the female(P=0.044).There ware significnat differences among histological subtypes in the EBERs detection rates(X* =8.224, P=0.004); nonkeratinizing carcinoma(76.9%),
undifferentiated carcinoma(68.7%), keratinizing squamous cell carcinoma(18.2%).
4) Seven(17.5%) of forty nasopharyngeal carcinoma specimens showed a LMP-specific staining with the monoclonal antibodies, CS1-4. All the seven cases were positive for EBERs by in situ hybridization. Anti-LMP antibody was localized to the cell
membrane
and cytoplasm of malignant epithelial cells. All the LMP positive cases were undifferentiated carcinoma, so that the LMP detection rate in undifferentiated carcinoma was 43.8%(7/16).
Above results implies that EBV infection is closely associated with the development of nasopharyngeal carcinoma. We suggest the EBERs transcript is an appropriate target for EBV detection with in situ hybridization technique in formalin-fixed
paraffin-embedded carcinoma specimens.
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KEYWORD
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